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How to trigger periplasmic release in recombinant Escherichia coli: A comparative analysis
Recombinant protein production in Escherichia coli usually leads to accumulation of the product inside the cells. To capture the product, cells are harvested, resuspended, and lysed. However, in cases where the product is transported to the periplasm, selective disruption of the outer membrane leads...
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| Publicado en: | Eng Life Sci |
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| Autores principales: | , , , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
John Wiley and Sons Inc.
2016
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6999231/ https://ncbi.nlm.nih.gov/pubmed/32624769 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/elsc.201600168 |
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