A highly sensitive trap vector system for isolating reporter cells and identification of responsive genes

We devised a versatile vector system for efficient isolation of reporter cells responding to a certain condition of interest. This system combines nontoxic GAL4-UAS and piggyBac transposon systems, allowing application to mammalian cells and improved expression of a fluorescent reporter protein for...

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Podrobná bibliografie
Vydáno v:Biol Methods Protoc
Hlavní autoři: Ishikawa, Kosuke, Kobayashi, Yuta, Wakabayashi, Yutaro, Watanabe, Shinya, Semba, Kentaro
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2018
Témata:
Methods Manuscript
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6994077/
https://ncbi.nlm.nih.gov/pubmed/32161797
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/biomethods/bpy003
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