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A fast, sensitive and cost-effective method for nucleic acid detection using non-radioactive probes

Nucleic acid detection and quantification using a labeled DNA probe is a very common molecular biology procedure. Here, we describe a new method, based on commonly used laboratory solutions, for nucleic acid hybridization and detection with digoxigenin-labeled DNA probes. The protocol described is f...

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Detalhes bibliográficos
Publicado no:Biol Methods Protoc
Main Authors: Viterbo, David, Marchal, Astrid, Mosbach, Valentine, Poggi, Lucie, Vaysse-Zinkhöfer, Wilhelm, Richard, Guy-Franck
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6994052/
https://ncbi.nlm.nih.gov/pubmed/32161800
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/biomethods/bpy006
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