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A UV cross-linking method combined with infrared imaging to analyse RNA–protein interactions

Photo cross-linking of proteins with short RNA oligomers is a classical method to study RNA–protein interactions that are implicated in many aspects of RNA metabolism and function. Most commonly, this involves the use of [γ-(32)P]-labeled RNA probes. Although very sensitive, these procedures are com...

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Detalhes bibliográficos
Publicado no:Biol Methods Protoc
Main Authors: Malmqvist, Tony, Spickett, Carl, Gallo, Jean-Marc, Anthony, Karen
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6994032/
https://ncbi.nlm.nih.gov/pubmed/32161791
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/biomethods/bpx009
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