Clonal rolling circle amplification for on-chip DNA cluster generation

Generation of monoclonal DNA clusters on a surface is a useful method for digital nucleic acid detection applications (e.g. microarray or next-generation sequencing). To obtain sufficient copies per cluster for digital detection, the single molecule bound to the surface must be amplified. Here we de...

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Publicat a:Biol Methods Protoc
Autors principals: Korfhage, Christian, Fricke, Evelyn, Meier, Andreas, Geipel, Andreas, Baltes, Mark, Krüger, Nadine, Herschel, Florian, Erbacher, Christoph
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2017
Matèries:
Methods Manuscript
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6994026/
https://ncbi.nlm.nih.gov/pubmed/32161790
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/biomethods/bpx007
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