Penetration model for chemical reactivation for resin-embedded green fluorescent protein imaging

In the so-called surface microscopy, serial block-face imaging is combined with mechanic sectioning to obtain volumetric imaging. While mapping a resin-embedded green fluorescent protein (GFP)-labeled specimen, it has been recently reported that an alkaline buffer is used to chemically reactivate th...

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Veröffentlicht in:J Biomed Opt
Hauptverfasser: Li, Longhui, Chen, Ruixi, Liu, Xiuli, Li, Ning, Liu, Xiaoxiang, Wang, Xiaojun, Quan, Tingwei, Lv, Xiaohua, Zeng, Shaoqun
Format: Artigo
Sprache:Inglês
Veröffentlicht: Society of Photo-Optical Instrumentation Engineers 2018
Schlagworte:
Special Section on Metabolic Imaging and Spectroscopy: Britton Chance 105th Birthday Commemorative
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6992894/
https://ncbi.nlm.nih.gov/pubmed/30484293
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1117/1.JBO.24.5.051406
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