EasyPrimer: user-friendly tool for pan-PCR/HRM primers design. Development of an HRM protocol on wzi gene for fast Klebsiella pneumoniae typing

In this work we present EasyPrimer, a user-friendly online tool developed to assist pan-PCR and High Resolution Melting (HRM) primer design. The tool finds the most suitable regions for primer design in a gene alignment and returns a clear graphical representation of their positions on the consensus...

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Publicat a:Sci Rep
Autors principals: Perini, Matteo, Piazza, Aurora, Panelli, Simona, Di Carlo, Domenico, Corbella, Marta, Gona, Floriana, Vailati, Francesca, Marone, Piero, Cirillo, Daniela Maria, Farina, Claudio, Zuccotti, Gianvincenzo, Comandatore, Francesco
Format: Artigo
Idioma:Inglês
Publicat: Nature Publishing Group UK 2020
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Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6987216/
https://ncbi.nlm.nih.gov/pubmed/31992749
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-57742-z
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