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Binding of Hemoglobin to Red Cell Membranes with Eosin-5-maleimide-Labeled Band 3: Analysis of Centrifugation and Fluorescence Lifetime Data

We have studied the binding of hemoglobin to the red cell membrane by centrifugation and fluorescence methods. The intact red cell was labeled with eosin-5-maleimide (EM), which specifically reacts with lysine 430 of band 3. Even though this residue is not part of the cytoplasmic domain of band 3 (c...

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Detalhes bibliográficos
Publicado no:Biochemistry
Main Authors: Demehin, Afolorunso Andrew, Abugo, Omoefe O., Jayakumar, Rajadas, Lakowicz, Joseph R., Rifkind, Joseph M.
Formato: Artigo
Idioma:Inglês
Publicado em: 2002
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6980380/
https://ncbi.nlm.nih.gov/pubmed/12093280
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi012007e
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