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A convenient strategy to clone small RNA and mRNA for high-throughput sequencing
High-throughput sequencing has become a standard tool for analyzing RNA and DNA. This method usually needs a cDNA/DNA library ligated with specific 5′ and 3′ linkers. Unlike mRNA, small RNA often contains modifications including 5′ cap or triphosphate and 2′-O-methyl, requiring additional processing...
Guardat en:
| Publicat a: | RNA |
|---|---|
| Autors principals: | , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Cold Spring Harbor Laboratory Press
2020
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6961543/ https://ncbi.nlm.nih.gov/pubmed/31754076 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.071605.119 |
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