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Tailoring cryo-electron microscopy grids by photo-micropatterning for in-cell structural studies
Spatially-controlled cell adhesion on electron microscopy (EM) supports remains a bottleneck in specimen preparation for cellular cryo-electron tomography. Here, we describe contactless and mask-free photo-micropatterning of EM grids for site-specific deposition of extracellular matrix (ECM)-related...
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| Publicado no: | Nat Methods |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2020
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6949126/ https://ncbi.nlm.nih.gov/pubmed/31740821 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41592-019-0630-5 |
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