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Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

The human gut is colonized by trillions of bacteria that support physiologic functions such as food metabolism, energy harvesting, and regulation of the immune system. Perturbation of the healthy gut microbiome has been suggested to play a role in the development of inflammatory diseases, including...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Shahi, Shailesh K., Zarei, Kasra, Guseva, Natalya V., Mangalam, Ashutosh K.
Formato: Artigo
Idioma:Inglês
Publicado em: 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6945761/
https://ncbi.nlm.nih.gov/pubmed/31680682
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/59980
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