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Sample Preparation by Easy Extraction and Digestion (SPEED) - A Universal, Rapid, and Detergent-free Protocol for Proteomics Based on Acid Extraction

The main challenge of bottom-up proteomic sample preparation is to extract proteomes in a manner that enables efficient protein digestion for subsequent mass spectrometric analysis. Today's sample preparation strategies are commonly conceptualized around the removal of detergents, which are ess...

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Bibliographic Details
Published in:Mol Cell Proteomics
Main Authors: Doellinger, Joerg, Schneider, Andy, Hoeller, Marcell, Lasch, Peter
Format: Artigo
Language:Inglês
Published: The American Society for Biochemistry and Molecular Biology 2020
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6944244/
https://ncbi.nlm.nih.gov/pubmed/31754045
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.TIR119.001616
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