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Exploring alternative catalytic mechanisms of Cas9 HNH domain

Understanding the reaction mechanism of Cas9 is crucial for the application of programmable gene editing. Despite the availability of the structures of Cas9 in apo- and substrate-bound forms, the catalytically active structure is still unclear. Our first attempt to explore the catalytic mechanism of...

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Detalhes bibliográficos
Publicado no:Proteins
Main Authors: Na Zhao, Li, Mondal, Dibyendu, Warshel, Arieh
Formato: Artigo
Idioma:Inglês
Publicado em: 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6942198/
https://ncbi.nlm.nih.gov/pubmed/31390092
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/prot.25796
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