In Silico and Experimental Evaluation of Primer Sets for Species-Level Resolution of the Vaginal Microbiota Using 16S Ribosomal RNA Gene Sequencing

BACKGROUND: Identification of bacteria in human vaginal specimens is commonly performed using 16S ribosomal RNA (rRNA) gene sequences. However, studies utilize different 16S primer sets, sequence databases, and parameters for sample and database clustering. Our goal was to assess the ability of thes...

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Bibliografiske detaljer
Udgivet i:J Infect Dis
Main Authors: Van Der Pol, William J, Kumar, Ranjit, Morrow, Casey D, Blanchard, Eugene E, Taylor, Christopher M, Martin, David H, Lefkowitz, Elliot J, Muzny, Christina A
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2019
Fag:
Major Articles and Brief Reports
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6941453/
https://ncbi.nlm.nih.gov/pubmed/30535155
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/infdis/jiy508
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