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Protocols for yTREX/Tn5‐based gene cluster expression in Pseudomonas putida

Bacterial gene clusters, which represent a genetic treasure trove for secondary metabolite pathways, often need to be activated in a heterologous host to access the valuable biosynthetic products. We provide here a detailed protocol for the application of the yTREX ‘gene cluster transplantation tool...

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Detalhes bibliográficos
Publicado no:Microb Biotechnol
Main Authors: Weihmann, Robin, Domröse, Andreas, Drepper, Thomas, Jaeger, Karl‐Erich, Loeschcke, Anita
Formato: Artigo
Idioma:Inglês
Publicado em: John Wiley and Sons Inc. 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6922528/
https://ncbi.nlm.nih.gov/pubmed/31162833
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/1751-7915.13402
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