High‐efficient and precise base editing of C•G to T•A in the allotetraploid cotton (Gossypium hirsutum) genome using a modified CRISPR/Cas9 system

The base‐editing technique using CRISPR/nCas9 (Cas9 nickase) or dCas9 (deactivated Cas9) fused with cytidine deaminase is a powerful tool to create point mutations. In this study, a novel G. hirsutum‐Base Editor 3 (GhBE3) base‐editing system has been developed to create single‐base mutations in the...

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Detalhes bibliográficos
Publicado no:Plant Biotechnol J
Main Authors: Qin, Lei, Li, Jianying, Wang, Qiongqiong, Xu, Zhongping, Sun, Lin, Alariqi, Muna, Manghwar, Hakim, Wang, Guanyin, Li, Bo, Ding, Xiao, Rui, Hangping, Huang, Huimei, Lu, Tianliang, Lindsey, Keith, Daniell, Henry, Zhang, Xianlong, Jin, Shuangxia
Formato: Artigo
Idioma:Inglês
Publicado em: John Wiley and Sons Inc. 2019
Assuntos:
Research Articles
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6920158/
https://ncbi.nlm.nih.gov/pubmed/31116473
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/pbi.13168
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