Hybridization-based capture of pathogen mRNA enables paired host-pathogen transcriptional analysis

Dual transcriptional profiling of host and bacteria during infection is challenging due to the low abundance of bacterial mRNA. We report Pathogen Hybrid Capture (PatH-Cap), a method to enrich for bacterial mRNA and deplete bacterial rRNA simultaneously from dual RNA-seq libraries using transcriptom...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Betin, Viktoria, Penaranda, Cristina, Bandyopadhyay, Nirmalya, Yang, Rui, Abitua, Angela, Bhattacharyya, Roby P., Fan, Amy, Avraham, Roi, Livny, Jonathan, Shoresh, Noam, Hung, Deborah T.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2019
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6917760/
https://ncbi.nlm.nih.gov/pubmed/31848386
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-55633-6
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