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A Targetron-Recombinase System for Large-Scale Genome Engineering of Clostridia

Clostridia are a group of Gram-positive anaerobic bacteria of medical and industrial importance for which limited genetic methods are available. Here, we demonstrate an approach to make large genomic deletions and insertions in the model Clostridium phytofermentans by combining designed group II int...

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Bibliografiska uppgifter
I publikationen:mSphere
Huvudupphovsmän: Cerisy, Tristan, Rostain, William, Chhun, Audam, Boutard, Magali, Salanoubat, Marcel, Tolonen, Andrew C.
Materialtyp: Artigo
Språk:Inglês
Publicerad: American Society for Microbiology 2019
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC6908422/
https://ncbi.nlm.nih.gov/pubmed/31826971
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/mSphere.00710-19
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