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Donor Fluorescence Decay Analysis for Energy Transfer in Double-Helical DNA with Various Acceptor Concentrations

We studied fluorescence resonance energy transfer between donors and acceptors bound to double-helical DNA. The donor Hoechst 33258 binds to the minor groove of DNA and the acceptor propidium iodide (PI) is an intercalator. The time-resolved donor decays were measured in the frequency domain. The do...

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Detalhes bibliográficos
Publicado no:Biopolymers
Main Authors: MURATA, SHIN-ICHI, KUŚBA, JÓZEF, PISZCZEK, GRZEGORZ, GRYCZYNSKI, IGNACY, LAKOWICZ, JOSEPH R.
Formato: Artigo
Idioma:Inglês
Publicado em: 2000
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6902054/
https://ncbi.nlm.nih.gov/pubmed/10958322
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/1097-0282(2000)57:5<306::AID-BIP70>3.0.CO;2-7
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