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Efficient Gene Disruption via Base Editing Induced Stop in Newt Pleurodeles waltl

Loss-of-function approaches provide strong evidence for determining the role of particular genes. The prevalent CRISPR/Cas9 technique is widely used to disrupt target gene with uncontrolled non-homologous end joining after the double strand breaks, which results in mosaicism and multiple genotypes i...

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Detalhes bibliográficos
Publicado no:Genes (Basel)
Main Authors: Cai, Hao, Peng, Zhelun, Ren, Ruimin, Wang, Heng
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6895984/
https://ncbi.nlm.nih.gov/pubmed/31652881
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/genes10110837
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