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Correcting Artifacts in Single Molecule Localization Microscopy Analysis Arising from Pixel Quantum Efficiency Differences in sCMOS Cameras

Optimal analysis of single molecule localization microscopy (SMLM) data acquired with a scientific Complementary Metal-Oxide-Semiconductor (sCMOS) camera relies on statistical compensation for its pixel-dependent gain, offset and readout noise. In this work we show that it is also necessary to compe...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Babcock, Hazen P., Huang, Fang, Speer, Colenso M.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6889274/
https://ncbi.nlm.nih.gov/pubmed/31792238
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-53698-x
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