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采用CRISPR/Cas9技术构建新品系HBeAg转基因小鼠
OBJECTIVE: To generate a new strain of HBeAg transgenic mice using CRISPR/Cas9 technique. METHODS: Hepatitis B virus (HBV) HBeAg gene was cloned and inserted in the pliver-HBeAg expression frame at the site of Rosa26 gene using CRISPR/Cas9 and homologous recombination techniques to construct the pli...
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| Pubblicato in: | Nan Fang Yi Ke Da Xue Xue Bao |
|---|---|
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
南方医科大学学报编辑部
2019
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6881734/ https://ncbi.nlm.nih.gov/pubmed/31640958 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.12122/j.issn.1673-4254.2019.09.03 |
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