Electropermeabilization-based fluorescence in situ hybridization of whole-mount plant parasitic nematode specimens

A fluorescence in situ hybridization (FISH) protocol was developed for nematodes in which nucleic acid probes are introduced within the organism via electroporation. This modification of existing FISH protocols removes numerous chemical wash steps, and thus, reduces protocol time and specimen loss w...

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Bibliographische Detailangaben
Veröffentlicht in:MethodsX
Hauptverfasser: Ruark-Seward, Casey L., Davis, Eric L., Sit, Tim L.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Elsevier 2019
Schlagworte:
Agricultural and Biological Science
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6881680/
https://ncbi.nlm.nih.gov/pubmed/31799140
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mex.2019.11.009
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