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Biotin Identification Proteomics in Three-Dimensional Organotypic Human Skin Cultures

Biotin identification (BioID) proteomics facilitates the unbiased detection of protein interaction neighborhoods in live cells. The BioID technique relies on the covalent biotin alteration of vicinal proteins by a modified bacterial biotin ligase. The biotin ligase is fused to a protein of interest...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Methods Mol Biol
Main Authors: Cable, Calvin J., Kaplan, Nihal, Getsios, Spiro, Thomas, Paul M., Perez White, Bethany E.
Format: Artigo
Jezik:Inglês
Izdano: 2020
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC6874900/
https://ncbi.nlm.nih.gov/pubmed/31123999
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/7651_2019_239
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