Characterization and repurposing of the endogenous Type I-F CRISPR–Cas system of Zymomonas mobilis for genome engineering

Application of CRISPR-based technologies in non-model microorganisms is currently very limited. Here, we reported efficient genome engineering of an important industrial microorganism, Zymomonas mobilis, by repurposing the endogenous Type I-F CRISPR–Cas system upon its functional characterization. T...

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Veröffentlicht in:Nucleic Acids Res
Hauptverfasser: Zheng, Yanli, Han, Jiamei, Wang, Baiyang, Hu, Xiaoyun, Li, Runxia, Shen, Wei, Ma, Xiangdong, Ma, Lixin, Yi, Li, Yang, Shihui, Peng, Wenfang
Format: Artigo
Sprache:Inglês
Veröffentlicht: Oxford University Press 2019
Schlagworte:
Synthetic Biology and Bioengineering
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6868425/
https://ncbi.nlm.nih.gov/pubmed/31647102
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkz940
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