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Implementation of interference reflection microscopy for label-free, high-speed imaging of microtubules
There are several methods for visualizing purified biomolecules near surfaces. Total-internal reflection fluorescence (TIRF) microscopy is a commonly used method, but has the drawback that it requires fluorescent labeling, which can interfere with the activity of the molecules. Also, photobleaching...
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| Publicado no: | J Vis Exp |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2019
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6858481/ https://ncbi.nlm.nih.gov/pubmed/31449260 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/59520 |
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