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Implementation of interference reflection microscopy for label-free, high-speed imaging of microtubules
There are several methods for visualizing purified biomolecules near surfaces. Total-internal reflection fluorescence (TIRF) microscopy is a commonly used method, but has the drawback that it requires fluorescent labeling, which can interfere with the activity of the molecules. Also, photobleaching...
Guardat en:
| Publicat a: | J Vis Exp |
|---|---|
| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2019
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6858481/ https://ncbi.nlm.nih.gov/pubmed/31449260 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/59520 |
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