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Temperature‐dependent irreversible conformational change of recombinant ADAMTS13 upon metal ion chelation

BACKGROUND: The catalytic domain of ADAMTS13 possesses one Zn(2+) and up to three putative Ca(2+) binding sites and can be inactivated by chelating agents. Although replenishment with an appropriate metallic cation is thought to restore the enzyme's proteolytic activity fully, ADAMTS13 stabilit...

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Detalhes bibliográficos
Publicado no:J Thromb Haemost
Main Authors: Rottensteiner, Hanspeter, Kaufmann, Stefan, Rathgeb, Anna, Kink, Barbara, Plaimauer, Barbara, Matthiessen, Peter, Hann, Stephan, Scheiflinger, Friedrich
Formato: Artigo
Idioma:Inglês
Publicado em: John Wiley and Sons Inc. 2019
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6850365/
https://ncbi.nlm.nih.gov/pubmed/31006963
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/jth.14440
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