Media improvement for 10 L bioreactor production of rPOXA 1B laccase by P. pastoris

In this work, we statistically improved culture media for rPOXA 1B laccase production, expressed in Pichia pastoris containing pGAPZαA-LaccPost-Stop construct and assayed at 10 L bioreactor production scale (6 L effective work volume). The concentrated enzyme was evaluated for temperature and pH sta...

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Pubblicato in:3 Biotech
Autori principali: Ardila-Leal, Leidy D., Albarracín-Pardo, Diego A., Rivera-Hoyos, Claudia M., Morales-Álvarez, Edwin D., Poutou-Piñales, Raúl A., Cardozo-Bernal, Angela M., Quevedo-Hidalgo, Balkys E., Pedroza-Rodríguez, Aura M., Díaz-Rincón, Dennis J., Rodríguez-López, Alexander, Alméciga-Díaz, Carlos J., Cuervo-Patiño, Claudia L.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Springer International Publishing 2019
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Original Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6848544/
https://ncbi.nlm.nih.gov/pubmed/31763125
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13205-019-1979-y
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