Quantifying the RNA cap epitranscriptome reveals novel caps in cellular and viral RNA

Chemical modification of transcripts with 5′ caps occurs in all organisms. Here, we report a systems-level mass spectrometry-based technique, CapQuant, for quantitative analysis of an organism's cap epitranscriptome. The method was piloted with 21 canonical caps—m(7)GpppN, m(7)GpppNm, GpppN, Gp...

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Publicat a:Nucleic Acids Res
Autors principals: Wang, Jin, Alvin Chew, Bing Liang, Lai, Yong, Dong, Hongping, Xu, Luang, Balamkundu, Seetharamsingh, Cai, Weiling Maggie, Cui, Liang, Liu, Chuan Fa, Fu, Xin-Yuan, Lin, Zhenguo, Shi, Pei-Yong, Lu, Timothy K, Luo, Dahai, Jaffrey, Samie R, Dedon, Peter C
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2019
Matèries:
Methods Online
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6847653/
https://ncbi.nlm.nih.gov/pubmed/31504804
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkz751
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