An Efficient and Economical Protocol for Isolating, Purifying and PEG-Mediated Transient Gene Expression of Chinese Kale Hypocotyl Protoplasts

In this study, we report the isolation and purification of protoplasts from Chinese kale (Brassica oleracea var. alboglabra) hypocotyls, and their transient gene expression transformation and subcellular localization of BaMYB75 (Bol042409). The upshot is that the vintage protocol included 5-d hypoco...

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Published in:Plants (Basel)
Main Authors: Sun, Bo, Yuan, Qiao, Zheng, Hao, Liang, Sha, Jiang, Min, Wang, Mei-Mei, Chen, Qing, Li, Meng-Yao, Zhang, Yong, Luo, Ya, Gong, Rong-Gao, Zhang, Fen, Tang, Hao-Ru
Format: Artigo
Language:Inglês
Published: MDPI 2019
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6843555/
https://ncbi.nlm.nih.gov/pubmed/31569422
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/plants8100385
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