Evaluation of 16S rRNA gene sequencing for species and strain-level microbiome analysis

The 16S rRNA gene has been a mainstay of sequence-based bacterial analysis for decades. However, high-throughput sequencing of the full gene has only recently become a realistic prospect. Here, we use in silico and sequence-based experiments to critically re-evaluate the potential of the 16S gene to...

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Udgivet i:Nat Commun
Main Authors: Johnson, Jethro S., Spakowicz, Daniel J., Hong, Bo-Young, Petersen, Lauren M., Demkowicz, Patrick, Chen, Lei, Leopold, Shana R., Hanson, Blake M., Agresta, Hanako O., Gerstein, Mark, Sodergren, Erica, Weinstock, George M.
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group UK 2019
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6834636/
https://ncbi.nlm.nih.gov/pubmed/31695033
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-019-13036-1
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