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Accessing Methyl Groups in Proteins via (1)H-detected MAS Solid-state NMR Spectroscopy Employing Random Protonation

We recently introduced RAP (reduced adjoining protonation) labelling as an easy to implement and cost-effective strategy to yield selectively methyl protonated protein samples. We show here that even though the amount of H(2)O employed in the bacterial growth medium is rather low, the intensities ob...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Asami, Sam, Reif, Bernd
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6828780/
https://ncbi.nlm.nih.gov/pubmed/31685894
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-52383-3
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