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A sensitive fluorescence-based assay to monitor enzymatic activity of the essential integral membrane protein Apolipoprotein N-acyltransferase (Lnt)

Lipoprotein modification is an essential process in Gram-negative bacteria. The action of three integral membrane proteins that catalyze the transfer of fatty acids derived from membrane phospholipids or cleave the signal peptide of the lipoprotein substrate result in the formation of mature triacyl...

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Publicat a:Sci Rep
Autors principals: Nozeret, Karine, Boucharlat, Alix, Agou, Fabrice, Buddelmeijer, Nienke
Format: Artigo
Idioma:Inglês
Publicat: Nature Publishing Group UK 2019
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6828757/
https://ncbi.nlm.nih.gov/pubmed/31685855
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-52106-8
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