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Fluorescence Anisotropy Measurements under Oxygen Quenching Conditions as a Method to Quantify the Depolarizing Rotations of Fluorophores. Application to Diphenylhexatriene in Isotropic Solvents and in Lipid Bilayers

We have measured the fluorescence anisotropy of 1,6-diphenyl-1,3,5-hexatriene (DPH) as its fluorescence lifetime is decreased by oxygen quenching. Such studies were done on DPH dissolved in the isotropic solvent mineral oil and for DPH embedded in phospholipid vesicles of either dimyristoyl-l-α-phos...

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Publicado en:Biochemistry
Autores principales: Lakowicz, J. R., Prendergast, F. G., Hogen, D.
Formato: Artigo
Lenguaje:Inglês
Publicado: 1979
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC6827200/
https://ncbi.nlm.nih.gov/pubmed/420798
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi00570a022
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