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Asymmetric Bilayers by Hemifusion: Method and Leaflet Behaviors
We describe a new method to prepare asymmetric giant unilamellar vesicles (aGUVs) via hemifusion. Hemifusion of giant unilamellar vesicles and a supported lipid bilayer, triggered by calcium, promotes the lipid exchange of the fused outer leaflets mediated by lipid diffusion. We used different fluor...
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| Publié dans: | Biophys J |
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| Auteurs principaux: | , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
The Biophysical Society
2019
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6818168/ https://ncbi.nlm.nih.gov/pubmed/31493862 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2019.07.054 |
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