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Modification of RSF1010-Based Broad-Host-Range Plasmids for Improved Conjugation and Cyanobacterial Bioprospecting

To facilitate the genetic engineering of diverse cyanobacterial strains, we have modified broad-host-range RSF1010-based plasmids to improve transmissibility, increase copy number, and facilitate cloning. RSF1010-based plasmids replicate in diverse bacterial strains but produce low amounts of useabl...

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Bibliografiske detaljer
Udgivet i:iScience
Main Authors: Bishé, Bryan, Taton, Arnaud, Golden, James W.
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2019
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6817606/
https://ncbi.nlm.nih.gov/pubmed/31585408
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.isci.2019.09.002
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