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Anisotropy-Based Sensing with Reference Fluorophores

We describe a new approach to fluorescence sensing based on measurements of steady-state anisotropies in the presence of reference fluorophores with known anisotropies. The basic concept is that the anisotropy of a mixture reflects a weighted average of the anisotropies of the emitting species. By u...

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發表在:Anal Biochem
Main Authors: Lakowicz, Joseph R., Gryczynski, Ignacy, Gryczynski, Zygmunt, Dattelbaum, Jonathan D.
格式: Artigo
語言:Inglês
出版: 1999
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC6816241/
https://ncbi.nlm.nih.gov/pubmed/10036147
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1006/abio.1998.3029
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