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Quantitation of TLR4 Internalization in Response to LPS in Thioglycollate Elicited Peritoneal Mouse Macrophages by Flow Cytometry

Internalization of cell surface Toll-like Receptor 4 (TLR4) is a convenient read-out to measure LPS dependent activation of the TRIF adaptor pathway. We here provide a protocol to quantify the LPS dependent internalization of TLR4 using thioglycollate-elicited peritoneal macrophages by flow cytometr...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Bio Protoc
Prif Awduron: Richard, Katharina, Vogel, Stefanie N, Perkins, Darren J
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Bio-Protocol 2019
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC6812529/
https://ncbi.nlm.nih.gov/pubmed/31649967
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3369
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