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C(18) Ceramide Analysis in Mammalian Cells Employing Reversed-Phase High Performance Liquid Chromatography Tandem Mass Spectrometry(a)

Ceramides play an important role in diverse cellular functions such as differentiation, cell cycle progression, cell-cell adhesion, senescence and apoptosis. Here we report a method of extracting lipids from mammalian cells and quantifying ceramide, where the assay conditions were optimized for repr...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Anal Biochem
Hauptverfasser: Haynes, Teka-Ann S., Duerksen-Hughes, Penelope J., Filippova, Maria, Filippov, Valery, Zhang, Kangling
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2008
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6812506/
https://ncbi.nlm.nih.gov/pubmed/18423390
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2008.03.045
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