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Determination of conjugated protein on nanoparticles by an adaptation of the Coomassie blue dye method

A new accurate spectrophotometric method for protein determination on nanoparticles is described. The method is based on the Coomassie blue dye that binds to the basic and aromatic amino acid residues of proteins, especially arginine and lysine. A known amount of reagent dye was mixed with a variety...

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Publicat a:MethodsX
Autors principals: Oviedo, Mariana J., Quester, Katrin, Hirata, Gustavo A., Vazquez-Duhalt, Rafael
Format: Artigo
Idioma:Inglês
Publicat: Elsevier 2019
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6812394/
https://ncbi.nlm.nih.gov/pubmed/31667112
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mex.2019.09.015
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