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Determination of conjugated protein on nanoparticles by an adaptation of the Coomassie blue dye method

A new accurate spectrophotometric method for protein determination on nanoparticles is described. The method is based on the Coomassie blue dye that binds to the basic and aromatic amino acid residues of proteins, especially arginine and lysine. A known amount of reagent dye was mixed with a variety...

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Detalhes bibliográficos
Publicado no:MethodsX
Main Authors: Oviedo, Mariana J., Quester, Katrin, Hirata, Gustavo A., Vazquez-Duhalt, Rafael
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2019
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6812394/
https://ncbi.nlm.nih.gov/pubmed/31667112
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mex.2019.09.015
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