Development of a quadriplex polymerase chain reaction for human cytomegalovirus detection

The development of a quadriplex PCR method with amplification of HCMV in a single‐step procedure using primers taken from four different regions of the viral genome is described. Different concentrations of dNTPs and MgCl(2) were assayed in order to optimize the constitution of the buffer for the mu...

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Detalhes bibliográficos
Publicado no:J Clin Lab Anal
Main Authors: Markoulatos, Panayotis, Samara, Vicky, Siafakas, Nikolaos, Plakokefalos, Elias, Spyrou, Niki, Moncany, Maurice L.J.
Formato: Artigo
Idioma:Inglês
Publicado em: John Wiley & Sons, Inc. 1999
Assuntos:
Original Articles
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6807959/
https://ncbi.nlm.nih.gov/pubmed/10323473
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/(SICI)1098-2825(1999)13:3<99::AID-JCLA2>3.0.CO;2-E
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