Quantitation of TGF‐β1 mRNA in porcine mesangial cells by comparative kinetic RT/PCR: Comparison with ribonuclease protection assay and in situ hybridization

Gene expression can be examined with different techniques including ribonuclease protection assay (RPA), in situ hybridisation (ISH), and quantitative reverse transcription–polymerase chain reaction (RT/PCR). These methods differ considerably in their sensitivity and precision in detecting and quant...

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Bibliographic Details
Published in:J Clin Lab Anal
Main Authors: Ceol, Monica, Forino, Monica, Gambaro, Giovanni, Sauer, Ulrich, Schleicher, Erwin D., D’Angelo, Angela, Anglani, Franca
Format: Artigo
Language:Inglês
Published: John Wiley & Sons, Inc. 2001
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Original Articles
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6807812/
https://ncbi.nlm.nih.gov/pubmed/11436205
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/jcla.1030
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