Quantitation of TGF‐β1 mRNA in porcine mesangial cells by comparative kinetic RT/PCR: Comparison with ribonuclease protection assay and in situ hybridization

Gene expression can be examined with different techniques including ribonuclease protection assay (RPA), in situ hybridisation (ISH), and quantitative reverse transcription–polymerase chain reaction (RT/PCR). These methods differ considerably in their sensitivity and precision in detecting and quant...

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Bibliografiske detaljer
Udgivet i:J Clin Lab Anal
Main Authors: Ceol, Monica, Forino, Monica, Gambaro, Giovanni, Sauer, Ulrich, Schleicher, Erwin D., D’Angelo, Angela, Anglani, Franca
Format: Artigo
Sprog:Inglês
Udgivet: John Wiley & Sons, Inc. 2001
Fag:
Original Articles
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6807812/
https://ncbi.nlm.nih.gov/pubmed/11436205
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/jcla.1030
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