One‐step heminested PCR for amplification of Neisseria meningitidis DNA in cerebrospinal fluid

A one‐step polymerase chain reaction (Heminested‐PCR) was designed to target the 16S rRNA fragment simultaneously using a set of primers for the universal bacterial group and a Neisseria meningitidis species‐specific sequence for diagnostic purposes. The diagnostic features of the Heminested‐PCR wer...

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Bibliografiske detaljer
Udgivet i:J Clin Lab Anal
Main Authors: Atobe, Jane H., Hirata, Mário H., Hoshino‐Shimizu, Sumie, Schmal, Manoel R., Mamizuka, Elsa M.
Format: Artigo
Sprog:Inglês
Udgivet: John Wiley & Sons, Inc. 2000
Fag:
Original Articles
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6807792/
https://ncbi.nlm.nih.gov/pubmed/10906773
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/1098-2825(2000)14:4<193::AID-JCLA9>3.0.CO;2-2
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