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CRISPR/Cas‐based screening of a gene activation library in Saccharomyces cerevisiae identifies a crucial role of OLE1 in thermotolerance

CRISPR/Cas‐based (clustered regularly interspaced short palindromic repeats/CRISPR‐associated) screening has been proved to be an efficient method to study functional genomics from yeast to human. In this study, we report the development of a focused CRISPR/Cas‐based gene activation library in Sacch...

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Publicat a:Microb Biotechnol
Autors principals: Li, Pengsong, Fu, Xiaofen, Zhang, Lei, Li, Shizhong
Format: Artigo
Idioma:Inglês
Publicat: John Wiley and Sons Inc. 2018
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6801138/
https://ncbi.nlm.nih.gov/pubmed/30394685
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/1751-7915.13333
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