Split-miniSOG for spatially detecting intracellular protein-protein interactions by correlated light and electron microscopy

A protein-fragment complementation assay (PCA) for detecting and localizing intracellular protein-protein interactions (PPIs) was built by bisection of miniSOG, a fluorescent flavoprotein derived from the light, oxygen, voltage (LOV)-2 domain of Arabidopsis phototropin. When brought together by inte...

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Veröffentlicht in:Cell Chem Biol
Hauptverfasser: Boassa, Daniela, Lemieux, Sakina P., Lev-Ram, Varda, Junru, Hu, Xiong, Qing, Phan, Sebastien, Mackey, Mason, Ramachandra, Ranjan, Peace, Ryan E., Adams, Stephen R., Ellisman, Mark H., Ngo, John T.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2019
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6800643/
https://ncbi.nlm.nih.gov/pubmed/31378710
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chembiol.2019.07.007
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