Optimized Protocol for the Detection of Multifunctional Epitope-Specific CD4(+) T Cells Combining MHC-II Tetramer and Intracellular Cytokine Staining Technologies

Analysis of multifunctional CD4(+) T cells is fundamental for characterizing the immune responses to vaccination or infection. Major histocompatibility complex (MHC)/peptide tetramers represent a powerful technology for the detection of antigen-specific T cells by specific binding to their T-cell re...

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Bibliographic Details
Published in:Front Immunol
Main Authors: Pastore, Gabiria, Carraro, Monica, Pettini, Elena, Nolfi, Emanuele, Medaglini, Donata, Ciabattini, Annalisa
Format: Artigo
Language:Inglês
Published: Frontiers Media S.A. 2019
Subjects:
Immunology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6794358/
https://ncbi.nlm.nih.gov/pubmed/31649661
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fimmu.2019.02304
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