Purification, kinetic characterization, and site-directed mutagenesis of Methanothermobacter thermautotrophicus RFAP Synthase Produced in Escherichia coli

Methane-producing archaea are among a select group of microorganisms that utilize tetrahydromethanopterin (H(4)MPT) as a one-carbon carrier instead of tetrahydrofolate. In H(4)MPT biosynthesis, β-ribofuranosylaminobenzene 5′-phosphate (RFAP) synthase catalyzes the production of RFAP, CO(2), and pyro...

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Detalhes bibliográficos
Publicado no:AIMS Microbiol
Main Authors: Bechard, Matthew E., Farahani, Payam, Greene, Dina, Pham, Anna, Orry, Andrew, Rasche, Madeline E.
Formato: Artigo
Idioma:Inglês
Publicado em: AIMS Press 2019
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6787355/
https://ncbi.nlm.nih.gov/pubmed/31663056
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3934/microbiol.2019.3.186
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