Isolation, purification, characterization and direct conjugation of the lipid A-free lipopolysaccharide of Vibrio cholerae O139

The lipopolysaccharide (LPS) of Vibrio cholerae O139, strain CIRS245, was isolated conventionally, and the lipid A was removed by mild acid hydrolysis (0.1 M NaOAc buffer containing 1% SDS, pH4.2, 95°C, 8 h). The crude product was a complex mixture consisting mainly of constituent fragments of the O...

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Опубликовано в: :Chemistry
Главные авторы: Xu, Peng, Korcová, Jana, Baráth, Peter, Čížová, Alžbeta, Valáriková, Jana, Qadri, Firdausi, Kelly, Meagan, O’Connor, Robert D., Ryan, Edward T., Bystrický, Slavomír, Kováč, Pavol
Формат: Artigo
Язык:Inglês
Опубликовано: 2019
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Article
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC6783332/
https://ncbi.nlm.nih.gov/pubmed/31306528
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/chem.201902263
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