Titrating Complex Mass Cytometry Panels

We describe here a simple and efficient antibody titration approach for cell‐surface markers and intracellular cell signaling targets for mass cytometry. The iterative approach builds upon a well‐characterized backbone panel of antibodies and analysis using bioinformatic tools such as SPADE. Healthy...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Cytometry A
Main Authors: Gullaksen, Stein‐Erik, Bader, Lucius, Hellesøy, Monica, Sulen, André, Fagerholt, Oda Helen Eck, Engen, Caroline B., Skavland, Jørn, Gjertsen, Bjørn Tore, Gavasso, Sonia
Format: Artigo
Jezik:Inglês
Izdano: John Wiley & Sons, Inc. 2019
Teme:
Technical Note
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC6766997/
https://ncbi.nlm.nih.gov/pubmed/30964237
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cyto.a.23751
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