An integrated workflow for crosslinking mass spectrometry

We present a concise workflow to enhance the mass spectrometric detection of crosslinked peptides by introducing sequential digestion and the crosslink identification software xiSEARCH. Sequential digestion enhances peptide detection by selective shortening of long tryptic peptides. We demonstrate o...

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Detalles Bibliográficos
Publicado en:Mol Syst Biol
Autores principales: Mendes, Marta L, Fischer, Lutz, Chen, Zhuo A, Barbon, Marta, O'Reilly, Francis J, Giese, Sven H, Bohlke‐Schneider, Michael, Belsom, Adam, Dau, Therese, Combe, Colin W, Graham, Martin, Eisele, Markus R, Baumeister, Wolfgang, Speck, Christian, Rappsilber, Juri
Formato: Artigo
Lenguaje:Inglês
Publicado: John Wiley and Sons Inc. 2019
Materias:
Methods
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC6753376/
https://ncbi.nlm.nih.gov/pubmed/31556486
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.15252/msb.20198994
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